MICROBIAL CONSORTIA

Richards Laboratories has used enrichment and selective cultivation techniques to obtain various microbial consortia with catabolic activities which allow bioremediation of environments heavily contaminated with recalcitrant and toxic organic compounds. Using these procedures we have developed the following consortia:

Gasoline digesters:This consortium grows in diphasic medium (gasoline/water) and mineralizes the components of gasoline to carbon dioxide and water.

Diesel digesters: This consortium has been adapted to degrade diesel fuels to carbon dioxide and water.

Phenol digesters: This consortium has been trained to not only tolerate extreme concentrations of phenol, but to also metabolize phenol, toluene, cresol and related compounds.

PAH digesters: This consortium readily metabolizes the lower molecular weight PAHs, such as naphthalene, phenanthrene, and anthracene. The higher molecular weight PAH’s such as chrysene and benzol-a-pyrene are bio-degraded at a lower rate.

Aromatic (BTEX) digesters: This consortium digests benzene, toluene, xylene’s, ethyl benzene, benzoate, phthalic acids, phenol, and cresols. These microbes grow in diphasic (toluene/water) medium.

This consortium can use oxygen or nitrate as the final electron acceptor.

Crude oil/ hydrocarbon digesters: This consortium produces a surfactant that disperses crude oils. The hydrocarbons are then digested. Nitrate or oxygen can be used as the final electron acceptor.

Starch digesters: This consortium produces strong amylase’s which hydrolyze starch polymers into mono and disaccharides which are then digested to carbon dioxide and water.

Chlorobenzene digesters. An anaerobic culture is used to convert completely chlorinated benzene to mono and di-chlorobenzene. An aerobic consortium then converts these chlorobenzene’s into carbon dioxide and water. The aerobic consortium will grow in a diphasic medium (di-chlorobenzene/water).

Other consortia that are available on request are: consortium for reductive dechlorination of polychlorinated biphenyls, consortium for co-metabolism of halogenated organic compounds, consortium for treatment of various chemical process waste waters, consortium for
bioremediation of chlorophenol wastes, consortium for treatment of coal tar-contaminated soils, consortium for food grease digestion, consortium for high energy compounds (TNT, PETN, RDX etc), consortium for digestion of ethylene and propylene glycols.

These cultures can be supplied either as fresh liquid cultures, dried on various substrates (such as corn starch) or freeze dried. The freeze dried cultures can be incorporated into pills on request.

Please contact our office to discuss any of your environmental consulting needs or just drop by and visit our office and laboratory in Pleasant Grove, Utah

Bacterial Culture before and After

PRODUCT DESCRIPTION – BACTERIAL DEGRADERS

BACTERIAL DEGRADERS

is a special mixture of bacteria designed for broad spectrum application on the industrial and waste water treatment field. It is designed to reduce BOD, COD, total suspended solids, treat phenolic wastes, bio-degrade hydrogen sulfide, and other various pollutants

SEPTIC DEGRADER

is a non-pathogenic bacterial product which contains a number of strains of bacteria. The bacteria populations have been determined by repetitive plate count to be in the excess of 350 million organisms per milliliter. The plate count will vary due to the cycle of organisms within the container from a low of 300 million organisms per milliliter to a high of 587 million organisms per milliliter.

The predominate organisms are the various strains of the purple bacteria group. This bacteria group consists of approximately 30 strains of bacteria which are sub-divided into two major categories, the purple sulfur bacteria and the purple non-sulfur organisms. The purple sulfur bacteria, also known as thiobacillus, utilize hydrogen sulfide as an electron donor and oxidizes hydrogen sulfide to elemental sulfur which is temporarily stored inter-cellularly. The elemental sulfur is then converted to sulfate by the bacteria and released. This metabolic process makes SEPTIC DEGRADER extremely efficient in removing odors due to hydrogen sulfide. The other organisms of the purple bacteria subgroup are the non-sulfur bacteria. These organisms are highly efficient at assimilating a wide range of organic compounds, including fatty acids, organic acids, primary and secondary alcohols, carbohydrates and many aromatic compounds. In addition, these organisms utilize carbon in their metabolic process. The non-sulfur purple bacteria group is about 95 per cent efficient at utilization of carbonaceous material. As a result, these organisms are effective at reducing carbonaceous BOD in waste streams.

SEPTIC DEGRADER also contains seven additional bacterial groups which are of proprietary nature. However, among these other organisms are species of Liquifaciens, Pseudomonas and other strains specifically cultured for application of this material to waste treatment. Over 90 per cent of the total bacteria contained in SEPTIC DEGRADERS are facultative in nature (i.e., they will thrive wither in the presence of free oxygen or are able to obtain oxygen from compounds containing oxygen). Together, the bacteria contained in this product, present a synergistic material capable of sustaining itself after packaging for a period of at least two years. The bacteria remain predominately in the adult stage, which affords SEPTIC DEGRADER the ability to readily adapt itself to various environments.

As a result of the make-up of this material, SEPTIC AND HYDROCARBON DEGRADERS have been used to treat highly complex organic compounds in a variety of treatment systems, including aerobic and anaerobic applications. They have also been successfully used for the treatment of phenolic wastes, high oil and grease, wastes for the control of odor due to hydrogen sulfide, and in other wastes which have proven to be effective.

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